Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Ultrafast Cooling With Total Liquid Ventilation Mitigates Early Inflammatory Response and Offers Neuroprotection in a Porcine Model of Cardiac Arrest

doi: 10.1161/JAHA.124.035617

Figure Lengend Snippet: Blood concentration of cytokines at baseline, 120 minutes, and 360 minutes after cardiac arrest in animals subjected to the control procedure or to rapid cooling with TLV. * P <0.05 vs corresponding baseline. † P <0.05 vs corresponding value at 120 minutes. CD31 indicates cluster of differentiation 31; IL, interleukin; MMP1, matrix metalloproteinase‐1; TLV, total liquid ventilation; and TNF‐alpha, tumor necrosis factor‐α.

Article Snippet: Briefly, cytokine concentrations were assessed using a dedicated porcine multiplex evaluating of TNF‐α (tumor necrosis factor‐α), IL‐1ra, IL‐6, IL‐8, IL‐10, IL‐18, MMP1 (matrix metalloproteinase‐1), and cluster of differentiation 31 (CD31) (8‐plex, number LXSAPM‐08, L151732; R&D Systems; Bio‐Plex 200; Bio‐Rad).

Techniques: Concentration Assay, Control

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Ultrafast Cooling With Total Liquid Ventilation Mitigates Early Inflammatory Response and Offers Neuroprotection in a Porcine Model of Cardiac Arrest

doi: 10.1161/JAHA.124.035617

Figure Lengend Snippet: Cytokine concentrations in the CSF at 120 minutes and 360 minutes after cardiac arrest in animals subjected to the control procedure or to rapid cooling with TLV as compared with sham animals neither subjected to cardiac arrest nor TLV. * P <0.05 vs control group. † P <0.05 vs sham. CD31 indicates cluster of differentiation 31; CSF, cerebrospinal fluid; IL, interleukin; MMP1, matrix metalloproteinase‐1; TLV, total liquid ventilation; and TNF‐alpha, tumor necrosis factor‐α.

Article Snippet: Briefly, cytokine concentrations were assessed using a dedicated porcine multiplex evaluating of TNF‐α (tumor necrosis factor‐α), IL‐1ra, IL‐6, IL‐8, IL‐10, IL‐18, MMP1 (matrix metalloproteinase‐1), and cluster of differentiation 31 (CD31) (8‐plex, number LXSAPM‐08, L151732; R&D Systems; Bio‐Plex 200; Bio‐Rad).

Techniques: Control

Journal: BioMed Research International

Article Title: Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property

doi: 10.1155/2019/3746326

Figure Lengend Snippet: Influence of myricetin on the production of inflammatory proteins and cytokines. (a) Influence of myricetin on the production of iNOS and COX-2 protein. HepG2 cells (1×106 cells) were precultured for 24 h and starved in serum-free medium for 2.5 h. The cells were then treated with the indicated concentrations of myricetin for 30 min and then exposed to 1 μ g/mL LPS for 12 h. The proteins of iNOS, COX-2, and α -tubulin were detected by Western blotting with their antibodies, respectively. (b) Myricetin decreased the levels of multiple inflammatory cytokines in HepG2 cells. The levels of 27 kinds of cytokines were measured by multiplex technology and bio-plex assay and arranged in an order from high to low change in the experimental inflammatory HepG2 cells. The data represent mean ± SD of four mice. ∗ p < 0.05 and ∗∗ p < 0.01. CTL, control; IL, interleukin; IFN- γ , interferon-gamma; TNF- α , tumor necrosis factor.

Article Snippet: The cells were then treated with 10-20 μ M of myricetin for half hour before exposure to 1 μ g/mL LPS for additional 12 h. The 27 cytokines detection, including TNF- α , G-CSF, IFN- γ , IL-1 α , IL-1 β , IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17, RANTES, Eotaxin, PDGF-BB, FGF basic, IP-10, MCP-1(MCAF), MIP-1 α , MIP-1 β , GM-CSF, and VEGF were performed by using Bio-Plex Pro Human Cytokine 27-Plex Panel kit (Bio-Rad Laboratories) and Bio-Plex cytokines assay system (Bio-Plex 200, Bio-Rad) according to the manufacturer's instructions and the results were analyzed by the Bio-Plex manager software (version 4.0).

Techniques: Western Blot, Multiplex Assay, Plex Assay, Control